Part:BBa_P10501:Design

GoldenBraid destination vector pDGB3 alpha1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 625
  Illegal XbaI site found at 392
  Illegal PstI site found at 404
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 625
  Illegal NheI site found at 3744
  Illegal PstI site found at 404
  Illegal NotI site found at 1619
  Illegal NotI site found at 3151
  Illegal NotI site found at 4441
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 625
  Illegal BamHI site found at 386
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 625
  Illegal XbaI site found at 392
  Illegal PstI site found at 404
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 625
  Illegal XbaI site found at 392
  Illegal PstI site found at 404
  Illegal NgoMIV site found at 1064
  Illegal NgoMIV site found at 1238
  Illegal NgoMIV site found at 1288
  Illegal NgoMIV site found at 1292
  Illegal NgoMIV site found at 1319
  Illegal NgoMIV site found at 1818
  Illegal NgoMIV site found at 2176
  Illegal AgeI site found at 4238
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 604
  Illegal BsaI.rc site found at 22
  Illegal SapI site found at 4832
  Illegal SapI.rc site found at 4369
  

Design Notes

More information to come

Source

More information to come